Bio-Guided Fractionation of Oil Palm (Elaeis guineensis) Fruit and Interactions of Compounds with First-Line Antituberculosis Drugs against Mycobacterium tuberculosis H37Ra
نویسندگان
چکیده
Natural products with antimycobacterial adjuvant potential may be utilized to address the rise of multidrug-resistant tuberculosis (TB). The antioxidant-rich oil palm (Elaeis guineensis) fruit (OPF) was investigated for activity against Mycobacterium (MTB) H37Ra using bio-guided fractionation techniques, followed by determination fractional inhibition index (FIC) first-line anti-TB drugs. In vitro screening microplate Alamar blue assay showed n-hexane and chloroform partitions OPF mesocarp had a minimum inhibitory concentration (MIC) 400–800 µg/mL. fraction contained nonanoic acid (C9H18O2), decanoic (C10H20O2), dodecanoic (C12H24O2), identified gas chromatography-mass spectrometry, which all an MIC 50 Nonanoic acids additive effects when combined streptomycin (FIC index: 0.625) rifampicin 0.75), respectively. Isoniazid 16-fold increase in acid. combination bactericidal 99.9% MTB Day 7 time-kill assay, structural damage cell wall observed electron microscopy. Cytotoxicity assessment Vero cells confirmed low toxicity LC50 > 200 shows presence fatty potential.
منابع مشابه
Circadian control of isoprene emissions from oil palm (Elaeis guineensis).
The emission of isoprene from the biosphere to the atmosphere has a profound effect on the Earth's atmospheric system. Until now, it has been assumed that the primary short-term controls on isoprene emission are photosynthetically active radiation and temperature. Here we show that isoprene emissions from a tropical tree (oil palm, Elaeis guineensis) are under strong circadian control, and that...
متن کاملMicrobial degradation of palm (Elaeis guineensis) biodiesel.
The kinetics of biodegradation of palm-derived fatty methyl and ethyl esters (Elaeis guineensis biodiesel) by a wild-type aerobic bacterial population was measured at 20 degrees C, as the rate of oxygen uptake by a manometric technique. The methyl and ethyl biodiesels were obtained by potassium-hydroxide catalysed trans-esterification of palm oil, respectively. The bacterial flora included the ...
متن کاملLipase activity, mesocarp oil content, and iodine value in oil palm fruits of Elaeis guineensis, Elaeis oleifera, and the interspecific hybrid O×G (E. oleifera × E. guineensis).
BACKGROUND One factor affecting crude palm oil quality is the formation of free fatty acids (FFA), often attributed to the hydrolytic action of mesocarp lipase. The aim of this work was to evaluate the enzyme behavior and to look toward new genotypes with low FFA production, high yield, and better oil quality. RESULTS Lipase activity was strongly activated at low temperatures (5 °C). At this ...
متن کاملStability analysis of oil yield in oil palm (Elaeis guineensis) progenies in different environments.
We evaluated 38 dura x pisifera (DP) oil palm progenies in four locations in Malaysia for genotype by environment interaction and genotypic stability studies. The DP progenies derived from crosses between pisifera palms of AVROS, Serdang S27B, Serdang 29/36, and Lever Cameroon were chosen to be the males' parent and Deli dura palms designated as females' parent. All the locations differed in te...
متن کاملStudy of Different Fertilization Methods on Oil Palm (Elaeis guineensis) Vegetative Factors
Oil palm propagation techniques are juvenile pot plants derived from germinated seeds and tissue culture, because it doesn’t produce sucker. In the nursery stage, monitoring the conditions of irrigation and nutrition are important to ensure phenotypic quality. During the nursery stage, optimal fertilization in development of more vigorous and productive plants. This research was carried out to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Separations
سال: 2021
ISSN: ['2297-8739']
DOI: https://doi.org/10.3390/separations8020019