Bio-Guided Fractionation of Oil Palm (Elaeis guineensis) Fruit and Interactions of Compounds with First-Line Antituberculosis Drugs against Mycobacterium tuberculosis H37Ra

نویسندگان

چکیده

Natural products with antimycobacterial adjuvant potential may be utilized to address the rise of multidrug-resistant tuberculosis (TB). The antioxidant-rich oil palm (Elaeis guineensis) fruit (OPF) was investigated for activity against Mycobacterium (MTB) H37Ra using bio-guided fractionation techniques, followed by determination fractional inhibition index (FIC) first-line anti-TB drugs. In vitro screening microplate Alamar blue assay showed n-hexane and chloroform partitions OPF mesocarp had a minimum inhibitory concentration (MIC) 400–800 µg/mL. fraction contained nonanoic acid (C9H18O2), decanoic (C10H20O2), dodecanoic (C12H24O2), identified gas chromatography-mass spectrometry, which all an MIC 50 Nonanoic acids additive effects when combined streptomycin (FIC index: 0.625) rifampicin 0.75), respectively. Isoniazid 16-fold increase in acid. combination bactericidal 99.9% MTB Day 7 time-kill assay, structural damage cell wall observed electron microscopy. Cytotoxicity assessment Vero cells confirmed low toxicity LC50 > 200 shows presence fatty potential.

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ژورنال

عنوان ژورنال: Separations

سال: 2021

ISSN: ['2297-8739']

DOI: https://doi.org/10.3390/separations8020019